to set up the software for your use. This guide shows a workflow that uses application settings. BD FACSDiva Software Quick Reference Guide for the BD LSR II. Compensation Controls in the BD FACSDiva Software Reference Manual. • Default templates are now provided for certain instrument functions. If you are the first user of the day: Boot up the computer and log on to Windows. Wait 10 seconds, then Open the BD FACSDiva software and log on. Allow the.
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Rename tubes, wells, and specimens. When the preference is selected, specify an export folder location by clicking the Browse button, or by entering a folder path in the Folder location field.
Populations with a drawing order of 1 are displayed in front of populations with a higher drawing order. Copy and paste wells to blank positions in the Plate window. For an example showing each scale method, see the following figure.
Menu bar Cytometer window Worksheet window toolbar Workspace toolbar Toolbar in Browser Current tube pointer Inspector Acquisition dashboard Status bar The following are brief descriptions of these components: The experiment modification date is automatically updated each time data in the experiment changes.
BD FACSDiva Software Reference Manual |
Plots cannot be resized below their original size. The y-axis scale shows either event counts or percentage of events in the histogram. This chapter contains information about instrument and acquisition controls that are common to all instruments. Click OK to dismiss the dialog box. Save Settings to Profile. The size of the plot doubles, making it easier to view individual events. By default, exported user profiles are stored at D: Use the Parameters tab to specify which parameter data should be sent and stored, to apply PMT amplification or electronic gain for FSCand to convert the parameter display to log.
Locked templates and any default templates provided with the software cannot be overwritten.
BD Biosciences facsdiv customer input on corrections and suggestions for improvement. In the Directory field, append the Practice file to D: Figure 10 shows the Cytometer Configuration window displaying the Configurations tab. Refer to your instrument manual for more information.
Zoom-In button—magnifies an area of a plot. Templates can be grouped by category so they are easier to find later.
Deselecting the checkbox will show the individual bins. Parameters are listed in the order they are defined in the Instrument Configuration dialog box. NOTICE If a plot displaying the Time parameter is hidden during acquisition or recording, no data will be shown for the time in which it was hidden.
Instrument and Acquisition Facsdivs Applying a Setup to Instrument Settings Saved setup values spectral overlap, threshold, and PMT voltages can be applied to an experiment, specimen, or tube, and spectral overlap values in a setup can be applied to a recorded tube. Tools for Data Analysis A summary of button functions follows. Note that specimens can be exported as templates only from open experiments. Use the Search field and buttons in the following ways: Six-color data is displayed in the plots.
Logs are named yyyy Month. No parts of this work may be reproduced in any form or by any means – graphic, electronic, or mechanical, including.
Log-density contours begin at the innermost contour using the peak height percentage you entered, and continue until they reach a threshold value of 1.
View experiment size in a Size column displayed in the browser. See Figure for an example of an unsmoothed plot.
BD FACSCanto II Quick Reference Manual
The icon turns green and the tube becomes the Active Tube in the Acquisition Dashboard. These plots facdiva similar to topographic maps which use contour lines to show points at the same elevation. Statistics are updated as the display changes.
For example, photodiode-generated pulses can be different from those generated by PMTs. You can search individual books, or search all books at once. Note that some buttons are shown only for certain cytometers. For example, fwcsdiva the Q1 cells would result in the following expression being sent to the cytometer: Text and keyboard conventions are shown in Table 2. These four tabs appear in the Inspector during offline use.
When performing a batch analysis with biexponential scales, start by setting the current tube pointer to the tube with facsdova negative range that best meets your laboratory s criteria.
Figure Selecting multiple objects selection handles on selected histogram main selected object selection handles on selected dot plot cursor showing object can be moved Individual selected softwade can also be resized by dragging a selection handle. Threshold and ratio settings are shown only if values have been entered in instrument settings.