Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Cultivo y fusion de protoplastos []. Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . El poder seleccionar eficientemente las plantas híbridas producidas a partir de fusión de protoplastos es fundamental. Se han logrado muchos avances en el.

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Protoplast fusion, culture and regeneration.

Plant Cell Rep 3: Due to that, fused and unfused protoplasts were cultured together. The latter fision a natural resistance to late blight, Phytophthora infestans Mont.

Fusion de protoplastos pdf

Nineteen plants were regenerated from calli Fig. Cubra la placa con una tapa. Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents. Literature cited Austin S. In this paper we describe the fusion of leaf protoplasts of S. Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S.

Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium. None of the plants had a similar banding pattern in relation to each one of the parents. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding.

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Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents. The numbers of chromosome of S. Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L. The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article. Please check your Internet connection and reload this page.

You must be signed in to post a comment. Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement. Protoplast-fusion derived Solanum cybrids: Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusion is independent of cell type. Non-transgenic applications of plant tissue culture in potato.

protoolastos A slot-blot hybridization method for screening somatic hybrids. Aggregations of more than two protoplasts were also observed. For the obtained somatic hybrid plants, the chromosome number varied from Table 2. Plant regeneration from mono- and dihaploid potato genotypes often results in ploidy changes, but little aneuploidy; on the other hand, regenerated plants from tetraploid genotypes produce a wide range of aneuploidy Kumar, Plant material and protoplast isolation.

Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae.

Cultivo y fusion de protoplastos [1990]

Con una cuchilla de afeitar nueva, retire la nervadura central de protoplastks hojas unifolioladas y luego corte los restos en 0. For other languages click here.


In Vitro Cell Dev. For this analysis, DNA extraction was performed according to Doyle and Doyle with some modifications. Interspecific somatic hybrids were obtained by polyethylene glycol fusion of protoplasts from tetraploid Solanum tuberosum L.

Cultivo y fusion de protoplastos

Gels were analyzed and photographed under UV light. Genetic Resourses Conservation and Characterization Division. Mantenga los protoplastos en el hielo durante 30 minutos.

The morphology of fusion-derived regenerants was intermediate between the donor parents. The origin of this chromosome instability has been studied elsewhere in potato Kumar, Centrifugar el tubo a x g por min a temperatura ambiente para cosechar protoplastos. Protoplasts fusion and plant regeneration. New York, NY Protoplasts fusion from S. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. After that, protoplasts settled down.

Instpp Cytological and RAPD marker analysis confirm their hybrid nature. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: Universidad Nacional Agraria La Molina.

The osmolality of this medium was fixed at mOsm. Regeneration in Gewebekulturen einiger Dihaploider Solanum tuberosum Z. Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L.