ImmunoCAP® ISAC and Microtest for multiplex allergen testing in people with difficult to manage allergic disease: a systematic review and cost analysis. A combination of innovative biochip technology with cutting edge research in molecular allergology has resulted in ImmunoCAP® ISAC – the most advanced in. The immuno-solid phase allergen chip (ISAC) test (Thermo Fisher ImmunoCAP ISAC) is a microarray assay system designed to assay specific.

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Specimen type to collect.

ImmunoCAP ISAC and Microtest for multiplex allergen testing | Guidance and guidelines | NICE

Owing to the paucity of available data, studies conducted in populations not specified as polysensitised or having difficult to manage allergic disease were also included.

Multiplex tests may be useful for investigating people with difficult to manage allergic disease: OFC testing was used as the reference standard. Full details of the characteristics of study participants, study inclusion and exclusion criteria, and intervention and comparator or reference standard are reported in the data extraction tables presented in Appendix 2 see Tables A—E. De Swert et al. An approved NPL form must accompany specimen. No studies of this type were identified.

The specific IgE chip delivers results for over a hundred components from more than 50 allergen sources.

Further details of the excluded full papers and the reasons for exclusion can be found in Appendix 4. Helping scientists meet the challenges they face in the lab or in the field every day. Unacceptable Conditions Ambient specimens.

Clear Turn Off Turn On. Change to diagnostic classification Two studies investigated the effect on diagnostic classification of adding ImmunoCAP ISAC testing to the standard diagnostic work-up of people with allergic disease.

ImmunoCAP ISAC Multiplexing – Phadia – Setting the Standard –

In general, single IgE performed similarly to SPT both measured whole extractswhereas ISAC gave much more variable results for the three different components measured. Change to management or treatment Two studies investigated the use of ImmunoCAP ISAC to guide decisions on the discontinuation of restrictive diets in children with food allergies. Full details of funding are reported in the baseline study details tables see Appendix 2Tables A—C.

This study 36 immunoczp only nine participants who received a total of 31 courses of SIT no details of diagnosis were reported. Site built and designed by Design. Comparator The comparator for this assessment was current standard care, which included allergy-focused clinical history, alternative tests of IgE antibody status single IgE antibody testingtests of clinical immuncoap such as skin prick testing isaf allergen challenge testing or a combination of these approaches.


Analyses to compare the effect of replacing single IgE with multiplex testing were difficult to perform because of lack of information regarding where the multiplex test would sit in the care pathway. Observational study designs were eligible for inclusion only if they reported measures of additional diagnostic information provided by multiplex allergen testing; studies that assessed only concordance between multiplex allergen testing and single IgE antibody testing or other tests were not included.

To assess the effects on treatment e. Study design There were no restrictions on study design.

ImmunoCAP ISAC 112 and Microtest for multiplex allergen testing

Clear Turn Off Turn On. Please review our privacy policy. Skin prick testing was used as the reference standard. The main potential sources of bias were in relation to participant selection and application of the index test. May include collection media, tubes, kits, etc. The systematic review component of this assessment found no data on the clinical consequences of adding multiplex allergen immmunocap to current clinical practice; therefore, a long-term economic model to inform health policy decision-making was not possible.

As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history. Single IgE testing and skin prick testing had comparable specificity See Stability for additional info.

No measure of the overall diagnostic performance of ISAC all components combined was reported. These will be reviewed by one of our allergy consultants in conjunction with your questionnaire, then sent to both you and to your GP if you opted for this in the questionnaire. For both methods, a positive result was defined as positive for at least one component or whole allergen; the cut-off values used to define positivity for individual components and allergens were not reported.

The principal investigators of completed trials identified through searches of clinical trials registries that appeared to meet our inclusion criteria but for which no publication could be identified were contacted and asked to provide publication details or unpublished data.


The sensitivities and specificities of the individual components ISAC 50 components were not reported. Turn recording iac on. isc

To assess the effects on clinical outcomes e. One study 39 was conducted in the UK, one study 38 was conducted in Italy and one study 36 did not report location.

A systematic review was conducted to summarise the evidence on the clinical effectiveness and cost-effectiveness of ImmunoCAP ISAC and Microtest for multiplex allergen testing in people with allergic disease. This issue may be freely reproduced for the purposes of private research and study and extracts or indeed, the full report may be included in professional journals provided that suitable acknowledgement is made and the reproduction is not associated with any form of advertising.

Diagnosis of food allergy De Swert et al. The inclusion criteria were expanded to allow studies that reported direct comparisons of diagnostic accuracy between single IgE testing and multiplex allergen testing, using SPTs or allergen challenge tests as the reference standard.

Background Multiplex allergen tests are molecular diagnostic tests, in the form of a glass slide, which can simultaneously test for the presence of multiple antibodies in blood samples up to 51 allergen sources.

Multiplex allergen tests are molecular diagnostic tests, in the form of a glass slide, which can simultaneously test for the presence of multiple antibodies in blood samples up to 51 allergen sources.

ImmunoCAP ISAC – When you need the bigger picture in allergy

No recommendations for service provision can be made based on the analyses included in this report. Additional specimen collection, transport, or test submission information. Results Clinical effectiveness A total of records were identified from searching and screened for inclusion. The diagnostic accuracy of Isax 50, used to measure the presence of one or more aeroallergens up to fivewas compared with the accuracy of single IgE tests of whole allergens.

The following key conference proceedings, were identified in consultation with clinical experts, and were screened for the last 5 years where available:. Directions to our clinics.